|
| Status |
Public on Jul 31, 2017 |
| Title |
H1299-DMSO |
| Sample type |
RNA |
| |
|
| Source name |
DMSO treated H1299 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H1299
|
| Treatment protocol |
H1299 cells were treated with either 20µM PD98059 (MEK inhibitor) or DMSO for 2 days
|
| Growth protocol |
Vector control and KRAS-G12V transfected HBEC3 cells were cultured in keratinocyte serum-free media supplemented with bovine pituitary extract and recombinant human EGF. H1299 cells were cultured in RPMI-1640 media supplemented with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the mirVANA miRNA isolation kit (Ambion, LifeTechnologies) as per manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were given to Genomics Division of the Iowa Institute of Human Genetics for miRNA profiling. Affymetrix GeneChip™ miRNA 4.0 Assay was utilized by the facility.
|
| |
|
| Hybridization protocol |
As per Affymetrix GeneChip™ miRNA 4.0 Assay
|
| Scan protocol |
Affymetrix GeneChip Model 3000 GeneChip Scanner was used for scanning
|
| Description |
miRNA profiling data from DMSO treated H1299 cells
|
| Data processing |
Data was processed using Affymetrix Expression Console and Transcriptome Analysis Console software 3.0.
|
| |
|
| Submission date |
Jul 06, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Charles Brenner |
| Organization name |
University of Iowa
|
| Department |
Biochemistry, Carver College of Medicine
|
| Lab |
4-339
|
| Street address |
Bowen Science Building, Newton Road
|
| City |
Iowa |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE100857 |
KRAS-driven miR-29b Expression is Required for Tumor Suppressor Gene Silencing |
|
