|
| Status |
Public on Nov 29, 2018 |
| Title |
HeLa_putrescine_5mM_day3 |
| Sample type |
RNA |
| |
|
| Source name |
HeLa, putrescine 5mM, day3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
treated with: Purtersine 5mM
time point: 72 hrs
|
| Treatment protocol |
HeLa cells were exposed to 0mM, 3mM and 5mM putrescine. Cell collection was performed at 24 hours and 72 hours.
|
| Growth protocol |
HeLa cells were incubated in DMEM which contains 10% dialized FBS and penicillin/ streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
ISOGEN was used for extracting total RNA
|
| Label |
Cy5
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
HeLa P5d3
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Jul 31, 2017 |
| Last update date |
Nov 29, 2018 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Street address |
10-1 tebiro 6-chome
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (1) |
| GSE102052 |
Gene expression profiles in HeLa cells exposed to putrescine |
|
