|
| Status |
Public on Aug 11, 2008 |
| Title |
CA2 Cre Sox17D |
| Sample type |
RNA |
| |
|
| Source name |
CA2, SOX17 expressing HESC - clone1
|
| Organism |
Homo sapiens |
| Characteristics |
Cell line established from CA2 HESC using pClip vector
|
| Treatment protocol |
For the establishment of transgenic populations, media was supplemented with Puromycin (Sigma, 80µg/mL) or G418 (Gibco, 100µg/mL). Cultures of 80% confluent cultures were harvested between passages 2 and 4 for microarray analysis.
|
| Growth protocol |
Control, SOX7 O/E and SOX17 O/E HESC were cultured under standard tissue culture conditions on mitotically inactivated mouse embryo fibroblast feeder layers in Knockout DMEM (Gibco) supplemented with 15% Serum Replacement (Gibco), Glutamax (Invitrogen), penicillin/streptomycin (Gibco), 1 mM non-essential amino acids (Gibco), 0.5mM mercaptoethanol, and 10ug/mL recombinant FGF2 (Peprotech).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA
|
| |
|
| Hybridization protocol |
15 ug of fragmented cRNA was used to hyb for 17hrs on Affymetrix Human genome U133 Plus 2.0 arrays
|
| Scan protocol |
GeneChip Scanner 3000 7G
|
| Description |
CA2 Cre Sox17D
|
| Data processing |
MAS 5.0, All GeneChips were scaled to a median intensity setting of 500 and CEL files normalized across all probe sets
|
| |
|
| Submission date |
Mar 13, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Cheryle Seguin |
| E-mail(s) |
[email protected]
|
| Organization name |
The Hospital for Sick Children
|
| Lab |
Rossant
|
| Street address |
101 College St
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE10809 |
Global gene expression from SOX7 and SOX17 over-expressing human embryonic stem cells (CA1 and CA2 lines) |
|
| Relations |
| Reanalyzed by |
GSE119087 |
