|
| Status |
Public on Sep 22, 2017 |
| Title |
PBMC+LPS_20 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC+LPS
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: peripheral mononuclear cells (PBMCs)
treated with: LPS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on microarray. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
SAMPLE 12
|
| Data processing |
The data were analyzed with R-Bioconductor packages using RMA algorithm.
|
| |
|
| Submission date |
Sep 21, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Celine Delucinge |
| Organization name |
University of Geneva
|
| Department |
iGE3 Genomics Platform
|
| Street address |
1 rue Michel Servet
|
| City |
Geneva |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL16043 |
| Series (2) |
| GSE104107 |
Effect of blood product - derived Cell Free Nucleic Acids (CFNA) on peripheral mononuclear cells gene regulation |
| GSE104109 |
Effect of cell free nucleic acids extracted from blood products on human leukocytes gene regulation: mononuclear cells and macrophages |
|
