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Sample GSM2792049 Query DataSets for GSM2792049
Status Public on Sep 25, 2019
Title HeLa_nonCSC, miRNA
Sample type RNA
 
Source name HeLa, nonCSC
Organism Homo sapiens
Characteristics cell line: HeLa
cell type: non-cancer stem cells
transfection: ZsGreen-cODCdegron
Growth protocol HeLa cells transfected with ZsGreen-cODCdegron were cultured in DMEM supplemented with 10% FBS and 1% P/S.
Extracted molecule total RNA
Extraction protocol ISOGEN was used for extracting total RNA
Label Cy5
Label protocol Extracted total RNA was labeled with Cy5 using the 3D-Gene miRNA labeling kit (Toray, Kamakura, Japan)
 
Hybridization protocol Hybridized for 16 h at 32 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
Scan protocol 3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
Data processing The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
 
Submission date Sep 25, 2017
Last update date Sep 25, 2019
Contact name Satoshi Kondo
Organization name Toray Industries,Inc.
Street address 10-1 tebiro 6-chome
City Kamakura
State/province Kanagawa
ZIP/Postal code 248-8555
Country Japan
 
Platform ID GPL18941
Series (2)
GSE104200 miRNA expression of HeLa cancer stem cells and non-cancer stem cells
GSE104201 mRNA and miRNA expression of HeLa cancer stem cells and non-cancer stem cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
MIMAT0000062 1967.7
MIMAT0000063 1298.9
MIMAT0000064 1594.2
MIMAT0000065 1572.7
MIMAT0000066 905.4
MIMAT0000067 1749.1
MIMAT0000068 193.8
MIMAT0000069 1488.8
MIMAT0000070 2462.5
MIMAT0000071 106.0
MIMAT0000072 184.8
MIMAT0000073 409.1
MIMAT0000074 1900.4
MIMAT0000075 2073.6
MIMAT0000076 8214.0
MIMAT0000077 294.6
MIMAT0000078 1762.2
MIMAT0000079 null
MIMAT0000080 1050.6
MIMAT0000081 233.3

Total number of rows: 2555

Table truncated, full table size 45 Kbytes.




Supplementary file Size Download File type/resource
GSM2792049_SH5XK24_Cell3.txt.gz 58.8 Kb (ftp)(http) TXT
Processed data included within Sample table

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