|
| Status |
Public on Sep 21, 2020 |
| Title |
LEC_7 |
| Sample type |
RNA |
| |
|
| Source name |
LECs of small intestines
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Small intestine
gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Up to 10,000 cells of target populations were sorted directly to Trizol reagent (Life Technologies). Total RNA was extracted in accordance with the manufacture's instruction.
|
| Label |
Cy3
|
| Label protocol |
Cyanine3-labeled cRNA from each sample was prepared subsequent to mRNA amplification by using Low Input Quick Amp Labeling Kit (one-color) (Agilent Technologies, Tokyo, Japan) according to the manufacture's instruction.
|
| |
|
| Hybridization protocol |
cRNA (600 ng) was hybridized to the microarray chip (SurePrint G3 Mouse Gene Expression v2 8x60K, Agilent Technologies).
|
| Scan protocol |
Slides were scanned immediately after washing on the SureScan Microarray Scanner (G2600D) using one color scan setting for 8x60k array slides (Scan resolution 3um, Dye channel is set to Green PMT sensitivity is set to 100%).
|
| Description |
Gene expression of VECs isolated from small intestines
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software (Agilent Technologies) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities.
|
| |
|
| Submission date |
Oct 15, 2017 |
| Last update date |
Sep 21, 2020 |
| Contact name |
Daigo Hashimoto |
| Organization name |
Hokkaido University
|
| Department |
Department of Hematology
|
| Street address |
N15W7 Kita-ku
|
| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
0608638 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE104979 |
Vascular endothelial cells (VECs) and lymphatic endothelial cells (LECs) of small intestines |
|
