|
| Status |
Public on Jun 17, 2008 |
| Title |
5262 UC Inflamed sigmoid colon |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
UC Inflamed sigmoid colon
|
| Organism |
Homo sapiens |
| Characteristics |
patient: 204
current medication: AZA 200mg, Insulin, Asprin, Atenolol, Simvastatin
birth date: 8/15/43
ethnicity: CAUCASIAN
symptoms onset date: 7/1/02
diagnosis date: 8/1/02
joint problems: FALSE
uc flare up: FALSE
family history: FALSE
aza treated: YES
aza tolerant: FALSE
aza at present: TRUE
procedure date: 11/1/04
indictation for procedure:
ucss: 5
calprotectin: 0
esr: 0
crp: 0
hb: 0
wcc: 0
neutrophils: 0
albumin: 0
blood obtained: FALSE
ibd affected relatives: 0
igr2096: 11
igr2198: 12
igr2230: 12
octn1: 12
octn2: 0
nod 702: 0
nod 908: 0
dlg5 133a: 0
nod 1007 fs: 0
diagnosis rectum: BOTH
diagnosis recto sigmoid: BOTH
diagnosis splen flex: BOTH
diagnosis hep flex: unknown
diagnosis total: unknown
last followup date: 11/1/04
followup rectum: BOTH
followup recto sigmoid: BOTH
followup splen flex: BOTH
followup hep flex: unknown
followup total: unknown
casenote review date: 12/16/04
category: FAILURE OF THERAPY
smoking status: ex
smoking start date: 1/1/58
smoking stop date: 1/1/01
smoking amount: 25+
other illnesses: Type 2 DM 02/2004.-Gout.
disease: UC
run_date: 1-25-01
anatomic_location: sigmoid colon
inflammation_status: Inflamed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
|
| Label |
Cy5
|
| Label protocol |
T7 RNA polymerase single round of linear amplification
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Human Reference RNA (Catalog #740000)
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: total RNA from 10 human cell lines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
|
| Label |
Cy3
|
| Label protocol |
T7 RNA polymerase single round of linear amplification
|
| |
|
| |
| Hybridization protocol |
18 hours at 60°C with constant rotation on Agilent WHG
|
| Scan protocol |
Agilent scanner according to the manufacturer’s protocol
|
| Description |
UC Inflamed sigmoid colon
|
| Data processing |
the data were extracted and normalized according to the default settings of Agilent Feature Extraction 7.5. LOWESS normalization, background subtraction, and ratio computation.
|
| |
|
| Submission date |
Apr 21, 2008 |
| Last update date |
Apr 12, 2017 |
| Contact name |
Alex Abbas |
| E-mail(s) |
[email protected]
|
| Organization name |
Genentech, Inc.
|
| Department |
Bioinformatics
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL1708 |
| Series (1) |
| GSE11223 |
Colon biopsies from UC patients and healthy controls |
|
