|
| Status |
Public on Nov 01, 2018 |
| Title |
CytoOrgs_IL13_Rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Intestinal organoid epithelial cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
treatment: treated with 20ng/ml IL-13
genotype: Wild-type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
3' polyA RNA seq
10X Genomics Chromium Prep
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
3' polyA RNA-seq
CellRanger 1.0
PCA (prcomp in R)
kNN-based graph clustering (R code)
Genome_build: mm10
Supplementary_files_format_and_content: 3' polyA RNA-seq. The expression levels for each gene (rows) in each single cell (column) in UMI counts as calculated using the CellRanger pipeline. Each column header provides the sequencing batch id, the cell barcode, and the cell type as identified by cluster analysis. Each ID is seperated by '_'
|
| |
|
| Submission date |
Nov 03, 2017 |
| Last update date |
Apr 05, 2019 |
| Contact name |
Adam Haber |
| E-mail(s) |
[email protected]
|
| Organization name |
Harvard T. H. Chan School of Public Health
|
| Street address |
665 Huntington Ave, HSPH Building 1, Room 305
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE106510 |
T helper cells modulate intestinal stem cell renewal and differentiation |
|
| Relations |
| BioSample |
SAMN07975621 |
| SRA |
SRX3360313 |
