|
| Status |
Public on Apr 10, 2018 |
| Title |
Murine myeloid precursors treated with CSF-1 alone 2 |
| Sample type |
RNA |
| |
|
| Source name |
Murine myeloid precursors_CSF-1 alone
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Black6
cell type: Murine enriched myeloid precurosrs
treated with: CSF-1 alone
|
| Treatment protocol |
Enriched myeloid precursors from mouse as well as Lonza Poietics human osteoclast precurosrs were seeded at 100,000 cells/ml in OCP-BlueKit (PT-8001, Lonza) according to manufacturer's protocol for indicated time.
|
| Growth protocol |
Murine bone marrow cells were flushed, grown on plastic plates with 100 ng/ml CSF-1 in DMEM supplemented with 10% FBS, L glutamine and 1X Pen-Strep for 3 days at 5% CO2 water jacketed incubator at 37 degree C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned in a GeneChip Scanner 3000 (Affymetrix Inc., USA)
|
| Description |
SS-4_CSF-1_1.CEL
|
| Data processing |
Gene expression levels were estimated from Expression console software from affymetrix (RMA-Gene-Core)
|
| |
|
| Submission date |
Nov 23, 2017 |
| Last update date |
Apr 10, 2018 |
| Contact name |
Michael C Ostrowski |
| E-mail(s) |
[email protected]
|
| Organization name |
Medical University of South Carolina
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Ostrowski
|
| Street address |
86 Jonathan Lucas street
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29425 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (2) |
| GSE107296 |
Bone density loci identified by genome-wide association studies segregate a lineage-specific PU.1-dependent gene regulatory network in osteoclasts [MoEx-1_0-st array] |
| GSE107297 |
Bone density loci identified by genome-wide association studies segregate a lineage-specific PU.1-dependent gene regulatory network in osteoclasts |
|
