|
| Status |
Public on Jun 21, 2018 |
| Title |
Opossum ESF MPA and PGE2 treated rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Endometrial stromal fibroblasts
|
| Organism |
Monodelphis domestica |
| Characteristics |
cell type: Endometrial stromal fibroblasts
treatment: MPA and PGE2
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Tissues were harvested and kept in RNAlater until extraction of RNA using Qiagen RNeasy extraction kit.
RNA libraries were prepared for sequencing using standard illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
MdESF_MPA_PGE2_2
|
| Data processing |
Basecalls performed using CASAVA
mRNA-seq reads were aligned to the opossum (monDom5) cDNA builds using Tophat2
Number of reads overlapped with known features (gene counts) was counted using HTSeq
transcripts per million (TPM) values were calculated according to Wagner et al (2012) Theor. Biosci.
Genome_build: opossum (monDom5)
Supplementary_files_format_and_content: tab-delimited txt files include read counts for each sample.
|
| |
|
| Submission date |
Jan 17, 2018 |
| Last update date |
Jun 21, 2018 |
| Contact name |
Cong Liang |
| E-mail(s) |
[email protected]
|
| Organization name |
Yale University
|
| Street address |
300 Heffernan Dr.
|
| City |
West Haven |
| ZIP/Postal code |
06516 |
| Country |
USA |
| |
|
| Platform ID |
GPL15381 |
| Series (1) |
| GSE109309 |
The mammalian decidual cell evolved from a cellular stress response |
|
| Relations |
| BioSample |
SAMN08370447 |
| SRA |
SRX3587686 |
