|
| Status |
Public on Jan 25, 2019 |
| Title |
MM.1S_drug |
| Sample type |
SRA |
| |
|
| Source name |
multiple myeloma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
cell type: B lymphoblast
treatment: 72 h after OR-S1 treatment
|
| Treatment protocol |
MM cells were treated with 1 μM OR-S1 for 72 h.
|
| Growth protocol |
RPMI1640 supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using an RNeasy Mini Kit (Qiagen).
Libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Avadis NGS Version 2.1 software was used for analysis.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg19 whole genome using Avadis NGS Version 2.1.
Genome_build: hg19
|
| |
|
| Submission date |
Jan 25, 2018 |
| Last update date |
Jan 25, 2019 |
| Contact name |
Makoto Nakagawa |
| E-mail(s) |
[email protected]
|
| Organization name |
Duke University
|
| Department |
Department of Orthopaedic Surgery
|
| Street address |
308 Research Drive
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE109673 |
Dual inhibition of EZH1/2 depletes stem cells and over-activates WNT signaling in multiple myeloma [RNA-seq] |
| GSE109675 |
Dual inhibition of EZH1/2 depletes stem cells and over-activates WNT signaling in multiple myeloma |
|
| Relations |
| BioSample |
SAMN08397416 |
| SRA |
SRX3600905 |
