Age: 12 yrs, when sample taken Gender: F Ethnicity: Hispanic or Latino Race: (Race Not Reported) Illness: SLE
Extracted molecule
total RNA
Extraction protocol
RNeasy Mini
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol
EukGE-WS2
Scan protocol
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 System.
Description
A female subject diagnosed with SLE where age 12 yrs, when sample taken. Sample used in Longitudinal study.
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.