|
| Status |
Public on Oct 14, 2018 |
| Title |
N2 500 mM NaCl replicate 4 |
| Sample type |
SRA |
| |
|
| Source name |
embryos
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: Bristol strain N2
tissue: embryos
growth condition: 500 mM NaCl in media
|
| Treatment protocol |
50 mM or 500mM NaCl.
|
| Growth protocol |
Wild-type, ssu-1(fc73), and daf-16(m26) embryos were placed on standard NGM plates.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 3 hrs, embryos were collected in M9, lysed using a BeadBug microtube homogenizer (Sigma) and 0.5 mm Zirconium beads (Sigma), and RNA was extracted using the RNeasy Mini kit (QIAGEN).
Illumina NeoPrep.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
D17-2264
mRNA
processed data file: nburton_fpkm.txt
|
| Data processing |
RSEM v. 1.2.15, with Bowtie v. 1.0.1 for read alignment (flags --paired-end -p 6 --bowtie-chunkmbs 1024 --forward-prob 0, for strand-specific libraries).
Genome_build: ce10 with 2015 Ensembl gene annotation.
Supplementary_files_format_and_content: nburton_fpkm.txt: Text file containing FPKM values generated by RSEM.
|
| |
|
| Submission date |
Feb 23, 2018 |
| Last update date |
Oct 14, 2018 |
| Contact name |
Charles Arthur Whittaker |
| E-mail(s) |
[email protected]
|
| Organization name |
Koch Institute
|
| Street address |
77 Mass Ave 76-189
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02152 |
| Country |
USA |
| |
|
| Platform ID |
GPL19757 |
| Series (1) |
| GSE111074 |
Neurohormonal signalling via a cytosolic sulfotransferase controls insulin sensitivity of C. elegans |
|
| Relations |
| BioSample |
SAMN08604372 |
| SRA |
SRX3739810 |
