|
| Status |
Public on Dec 31, 2008 |
| Title |
Human-LMD dissected breast cancer 5B |
| Sample type |
RNA |
| |
|
| Source name |
human-LMD dissected breast cancer estradiol conc.:10.5pg/g
|
| Organism |
Homo sapiens |
| Characteristics |
Gender:female
Tissue:human-LMD dissected breast cancer
Type:Invasive ductal carcinoma
Tissue estradiol conc.:10.5pg/g
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Mini Kit(Qiagen) according to the instruction manual
|
| Label |
biotin
|
| Label protocol |
The standard protocol described in Affymetrix GeneChip expression analysis manual.
|
| |
|
| Hybridization protocol |
The standard protocol described in Affymetrix GeneChip expression analysis manual.
|
| Scan protocol |
The standard protocol described in Affymetrix GeneChip expression analysis manual.
|
| Description |
Breast carcinoma were embedded in Tissue-Tek optimal cutting temperature compound(Sakura Finetechnical Co.Ltd., Tokyo, Japan) and frozen sectioned at a thickness of 8 micro mm. Approximately 5000 cells were laser transferred from the carcinoma tissue and applied to microarray analysis. Each population of the cells was estimated to be >98% homogeneous as determined by microscopic visualization of the captured cells.
Tissue estradiol concentrations were measured by liquid chromatography/electrospray tandem mass spectrometry (LC-MS/MS) analysis.
|
| Data processing |
Data processing was performed according to Affymetrix GeneChip expression analysis manual. Expression signals were extracted and normalized using MAS5.0.
|
| |
|
| Submission date |
Jul 02, 2008 |
| Last update date |
Jul 03, 2008 |
| Contact name |
Shuji Nagasaki |
| Organization name |
Tohoku University Graduate School of Medicine
|
| Street address |
2-1 Seiryo-machi, Aoba-ku
|
| City |
Sendai |
| State/province |
Miyagi-ken |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE11965 |
Contribution of HSD17B12 for estradiol biosynthesis in human breast cancer |
|
