|
| Status |
Public on Aug 23, 2018 |
| Title |
Calu-3 SIP/Calu-3 control |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Calu-3 control
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: bronchial epithelial cell
cell line: Calu-3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA
|
| Label |
Cy5
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Channel 2 |
| Source name |
Calu-3 SIP
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: bronchial epithelial cell
cell line: Calu-3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA
|
| Label |
Cy3
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| |
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Data processing |
F635 Median column as Red signal and F532 Median column as Green signal. Global normalized, background subtracted data obtained from log2 of processed Green signal/processed Red signal.
|
| |
|
| Submission date |
Mar 13, 2018 |
| Last update date |
Aug 24, 2018 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Street address |
10-1 tebiro 6-chome
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (1) |
| GSE111746 |
The effect of S1P on the gene expression in the bronchial epithelial cells |
|
