|
| Status |
Public on Feb 17, 2009 |
| Title |
Brain_r3 |
| Sample type |
RNA |
| |
|
| Source name |
Stratagene human brain total RNA
|
| Organism |
Homo sapiens |
| Characteristics |
Brain
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
NA
|
| Label |
Cy3
|
| Label protocol |
Each sample was prepared according to the Agilent’s miRNA Microarray System protocol. 100 ng of total RNA was dephosphorylated with calf intestine alkaline phosphatase (GE Healthcare Europe GmbH, Otelfingen, Switzerland), denatured with dimethyl sulfoxide and labeled with pCp-Cy3 using T4 RNA ligase (GE Healthcare Europe GmbH, Otelfingen, Switzerland).
|
| |
|
| Hybridization protocol |
The labeled RNA was hybridized to Agilent human miRNA microarrays for 20 hours at 55°C with rotation.
|
| Scan protocol |
The arrays were scanned with an Agilent microarray scanner with high dynamic range settings as specified by the manufacturer.
|
| Description |
Technical replicate 3 (labeling and hybridization)
|
| Data processing |
Agilent Feature Extraction Software was used to extract the data and different normalization procedures were applied to the Total Gene Signal intensity values
|
| |
|
| Submission date |
Jul 11, 2008 |
| Last update date |
Jan 29, 2010 |
| Contact name |
Sylvain Pradervand |
| E-mail(s) |
[email protected]
|
| Phone |
+41 21 692 39 08
|
| Organization name |
UNI Lausanne
|
| Department |
CIG
|
| Lab |
DNA Array Facility
|
| Street address |
Genopode
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL9081 |
| Series (1) |
| GSE12085 |
Impact of normalization on Agilent miRNA microarray expression profiling |
|
