|
| Status |
Public on Mar 15, 2019 |
| Title |
30T |
| Sample type |
RNA |
| |
|
| Source name |
organoids established using cancer tissue but failed in long culture
|
| Organism |
Homo sapiens |
| Characteristics |
tissue/cell type: organoids established using cancer tissue but failed in long culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted from tissues of organoids using miRVana isolation kit.
|
| Label |
Cy5
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Mar 27, 2018 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Street address |
10-1 tebiro 6-chome
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (2) |
| GSE112407 |
Gene expression profiles in organoids derived from biliary tract carcinomas [MAA-17164_3] |
| GSE112408 |
microRNA and geneexpression profiles in organoids derived from biliary tract carcinomas |
|
