|
| Status |
Public on Jul 26, 2008 |
| Title |
Alveolar macrophages, non-smoker 007, B. cepacia BC treatment |
| Sample type |
RNA |
| |
|
| Source name |
alveolar macrophages obtained from bronchoalveolar lavage
|
| Organism |
Homo sapiens |
| Characteristics |
Age: 38
Sex: M
Ethnic group: black
Smoking status: smoker, 14 pack-years
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA for HuGeneFL (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HuGeneFL. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using GeneArray 2500.
|
| Description |
Alveolar macrophages, non-smoker 007, B. cepacia BC treatment
|
| Data processing |
MAS 4
|
| |
|
| Submission date |
Jul 25, 2008 |
| Last update date |
Jul 25, 2008 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
[email protected]
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Department of Genetic Medicine
|
| Lab |
Crystal
|
| Street address |
1300 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL80 |
| Series (1) |
| GSE12245 |
Similarity of Gene Expression Patterns in Human Alveolar Macrophages in Response to P. aeruginosa and B. cepacia |
|
