|
| Status |
Public on Sep 19, 2008 |
| Title |
B2KO; Isoproterenol-treated #4 |
| Sample type |
RNA |
| |
|
| Source name |
Heart
|
| Organism |
Mus musculus |
| Characteristics |
10 weeks old
|
| Treatment protocol |
Mice of each genotype were randomly assigned to fourteen day infusions of isoproterenol (120 µg/g/day) or no treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Twenty WT FVB, WT C57, and B2KO mice were given intraperitoneal injections of avertin (100μl/10g body weight) and sacrificed by cervical dislocation. Half of the mice in each group had undergone fourteen day isoproterenol infusions. RNA was extracted from the mouse hearts using Quiagen RNeasy® Midi kit. Samples were homogenized in buffer containing guanidine isothiocyanate and beta mercaptoethanol. Ethanol was then added to the lysate. The sample was then applied to the RNeasy column. RNA was eluted from the column using 80 ul of RNase-free water.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip 430-2 Mouse Gene array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Genechip Scanner 3000.
|
| Description |
Gene expression data from mice heart
|
| Data processing |
GC-RMA
|
| |
|
| Submission date |
Aug 11, 2008 |
| Last update date |
Sep 18, 2008 |
| Contact name |
Rani Agrawal |
| E-mail(s) |
[email protected]
|
| Phone |
(650) 726-0355
|
| Organization name |
Stanford University
|
| Department |
Anesthesia
|
| Lab |
Patterson Lab
|
| Street address |
300 Pasteur Drive, Dept of Anesthesia
|
| City |
stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE12413 |
Prediction of left ventricle systolic dysfunction in mice using gene expression profiling |
|
