|
| Status |
Public on Jan 10, 2019 |
| Title |
ctrl DamPol2 |
| Sample type |
SRA |
| |
|
| Source name |
ctrl DamPol
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: Fuss expressing neurons
genotype: tubgal80ts/DamPol; fussdelds-Gal4/+
age: 1-3 day old adult flies
gender: pooled male and female
sample composition: 50 males, 50 females
|
| Growth protocol |
development at 18°C. 1-3 day old flies were shifted to 29°C for 24h.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Flies were snapfrozen in liquid nitrogen and heads were removed by vortexing and sieving. Further procedures where executed as described in Southall et al. (2016)
libraries were prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
Processed data file: ctrl.gatc.average.bedgraph
|
| Data processing |
Library strategy: DamID
.bam files are converted to .fastq files and were gunzipped
log2(DamPolII/Dam) in bedgraph format have been generated by DamIDseq_pipeline provided by Marshall and Brand (2015)
single datasets have been combined and averaged to one single log2(DamPolII/Dam) per genotype with the average_tracks script provided by Marshall and Brand (2015)
Genome_build: dm6
Supplementary_files_format_and_content: bedgraph files generated
|
| |
|
| Submission date |
Jun 05, 2018 |
| Last update date |
Jan 10, 2019 |
| Contact name |
Mathias Rass |
| Organization name |
University of Regensburg
|
| Department |
Developmental Biology
|
| Street address |
Universitätsstraße 31
|
| City |
Regensburg |
| ZIP/Postal code |
93040 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23323 |
| Series (1) |
| GSE115347 |
Targeted DamID reveals Fuss as a pivotal element in bitter gustatory neurons |
|
| Relations |
| BioSample |
SAMN09361163 |
| SRA |
SRX4167522 |
