|
| Status |
Public on Sep 05, 2009 |
| Title |
S_H_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic heart
|
| Organism |
Gallus gallus |
| Characteristics |
the embryonic heart of Shouguang Chicken in hypoxia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The extraction of total RNA with TRIzol reagent was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 microg total RNA (Expression Analysis Technical Manual,P/N900223 or 90365, Affymetrix).
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized to Affymetrix GeneChip® Chicken Genome Array for 16 h at 45°C. The hybridized probe array was automatically washed and then stained with streptavidin-phycoerythrin (SAPE) in a GeneChip® Fluidics Station 450.
|
| Scan protocol |
The arrays were scanned on a Affymetrix GeneChip® Scanner 3000.
|
| Description |
Gene expression data from the embryonic heart of Shouguang Chicken in hypoxia.
|
| Data processing |
The data were analyzed by GeneChip® Operating Software (GCOS) with Affymetrix default analysis settings.
|
| |
|
| Submission date |
Sep 05, 2008 |
| Last update date |
Sep 05, 2008 |
| Contact name |
mei li |
| E-mail(s) |
[email protected]
|
| Organization name |
China Agricultural University
|
| Street address |
Haidian district
|
| City |
Beijing |
| ZIP/Postal code |
86100193 |
| Country |
China |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE12675 |
Hypoxia effect on embryonic hearts of Tibetan Chicken, Dwarf Recessive White Chicken and Shouguang Chicken |
|
