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Sample GSM335236 Query DataSets for GSM335236
Status Public on Oct 23, 2008
Title ALL_responsive_4-IR
Sample type RNA
 
Source name ALL, responsive, no irradiation
Organism Homo sapiens
Characteristics B-precursor ALL tumour
responsive to IR-induced DNA damage
female
Treatment protocol Cells were irradiated and maintained in culture for 8 hours, or maintained for 8 h without irradiation.
Growth protocol Mononuclear cells were isolated from bone marrow aspirate using Ficoll-Paque (Amersham Biosciences, Piscataway, NJ), and cells were cultured in RPMI 1640. Only samples with more than 90% of blast cells were used for analysis.
Extracted molecule total RNA
Extraction protocol Standard Affymetrix protocol
Label Biotin
Label protocol Standard Affymetrix protocol
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description 89_0h
Data processing Data for all arrays was normalised in the GeneSpring GX 7.3.1 analysis package to the 50th percentile of total expression value data for each individual chip, and to the median for each gene across arrays (default parameters).
 
Submission date Oct 20, 2008
Last update date Oct 23, 2008
Contact name Carmel M McConville
E-mail(s) [email protected]
Organization name University of Birmingham
Department School of Cancer Sciences
Street address Vincent Drive
City Birmingham
State/province UK
ZIP/Postal code B15 2TT
Country United Kingdom
 
Platform ID GPL96
Series (1)
GSE13280 Gene expression analysis of paediatric acute lymphoblastic leukaemia

Data table header descriptions
ID_REF
VALUE GeneSpring normalized data

Data table
ID_REF VALUE
1007_s_at 1.417
1053_at 1.34
117_at 0.255
121_at 0.941
1255_g_at 1.293
1294_at 0.852
1316_at 1.037
1320_at 1.94
1405_i_at 0.303
1431_at 0.906
1438_at 0.83
1487_at 0.884
1494_f_at 1.155
1598_g_at 0.999
160020_at 1.366
1729_at 0.601
177_at 0.209
1773_at 0.837
179_at 0.976
1861_at 1.468

Total number of rows: 22283

Table truncated, full table size 366 Kbytes.




Supplementary file Size Download File type/resource
GSM335236.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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