|
| Status |
Public on Apr 23, 2010 |
| Title |
control, biological rep5 |
| Sample type |
RNA |
| |
|
| Source name |
normal Lung tissue, control, biological rep5
|
| Organism |
Homo sapiens |
| Characteristics |
Normal lung tissues obtained from the disease free margins with normal histology of lung cancer resection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy mini kit according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled RNA was prepared according to the standard Agilent protocol from 100ng total RNA
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized for 20 hr at 55C on Agilent 8X15K microRNA array. Arrays were washed according to the manufacturer's protocol.
|
| Scan protocol |
Scanned on an Agilent G2505A Scanner
|
| Description |
Lung tissue samples obtained from the University of Pittsburgh Health Sciences Tissue Bank. miRNA expression data from a lung with usual interstitial pneumonia.
|
| Data processing |
Agilent Feature Extraction v9.5.3, default miRNA parameters
|
| |
|
| Submission date |
Oct 23, 2008 |
| Last update date |
Apr 23, 2010 |
| Contact name |
Kusum Vijay Pandit |
| E-mail(s) |
[email protected]
|
| Phone |
412-624-9046
|
| Organization name |
University of Pittsburgh
|
| Department |
Medicine-PACCM
|
| Street address |
200, Lothrop street, W1200-25A Biomedical Science Tower
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL6955 |
| Series (1) |
| GSE13316 |
miRNA expression data in idiopathic pulmonary fibrosis |
|
