|
| Status |
Public on Jan 01, 2019 |
| Title |
C564_IBIN_Ecl6_B |
| Sample type |
SRA |
| |
|
| Source name |
whole organism
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: IBIN;C564-GAL4
infect: Enterobacter cloacae
infection time: 6 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNAs were extracted with TRI reagent according to the manufacturers protocol (ThermoFisher Scientific) and the resulting RNAs treated with the RapidOut DNA Removal Kit (ThermoFisher Scientific).
Library preparation was done according to Illumina TruSeq® Stranded mRNA Sample Preparation Guide (part # 15031047)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
sequencing was done in 1 lane
|
| Data processing |
The base calling was done using bcl2fastq (v. 2.17)
Read alignment was done using star (2.5.2b) to dm6 Drosophila melanogaster genome reference
Genewise read counts were calculated using subread (v1.5.1)
Genome_build: Drosophila melanogaster dm6
Supplementary_files_format_and_content: files are tab delimited text files containing the genewise read counts for each sample
|
| |
|
| Submission date |
Sep 25, 2018 |
| Last update date |
Jan 02, 2019 |
| Contact name |
Mika Ramet |
| E-mail(s) |
[email protected]
|
| Organization name |
Tampere University
|
| Department |
Faculty of Medicine and Health Technology
|
| Lab |
Experimental Immunology
|
| Street address |
Arvo Ylpön katu 34
|
| City |
TAMPERE |
| ZIP/Postal code |
33520 |
| Country |
Finland |
| |
|
| Platform ID |
GPL23323 |
| Series (1) |
| GSE120437 |
Overexpression of IBIN (CG44404) using C564-GAL4 driver enhances a group of Toll pathway target genes and shows effects on metabolism |
|
| Relations |
| BioSample |
SAMN10127653 |
| SRA |
SRX4737425 |
