|
| Status |
Public on Sep 01, 2009 |
| Title |
2 hour PE1.3 embolized rat, lung tissue, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Total lung tissue 2 hours after treatment
|
| Organism |
Rattus norvegicus |
| Characteristics |
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
| Treatment protocol |
Rat was injected in the right jugular vein with 0.10 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE1.3 treatment; 1.3 million microspheres/100 gram weight
|
| Growth protocol |
Rat was euthanized 2 hours after PE1.3 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
|
| |
|
| Hybridization protocol |
25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
|
| Description |
Gene expression data from total lung RNA after 2 hours of PE1.3 treatment.
|
| Data processing |
The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
|
| |
|
| Submission date |
Nov 08, 2008 |
| Last update date |
Mar 30, 2009 |
| Contact name |
John Zagorski |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
704-355-3663
|
| Organization name |
Carolinas Medical Center
|
| Department |
Emergency Medicine
|
| Lab |
Cannon Research Center
|
| Street address |
1542 Garden Terrace, 301
|
| City |
Charlotte |
| State/province |
NC |
| ZIP/Postal code |
28203 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE13535 |
Rat lung gene expression during acute pulmonary embolism |
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