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Sample GSM341695 Query DataSets for GSM341695
Status Public on Jan 22, 2009
Title Expression data from BAT of the KRAP deficient mice (WT2)
Sample type RNA
 
Source name brown adipose tissue
Organism Mus musculus
Characteristics C57BL6J-backcrossed, male
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA followed by purification using Rneasy kit was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description WT2-BAT
Data processing The data were analyzed with GeneChip Operating Software (GCOS) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Nov 13, 2008
Last update date Aug 28, 2018
Contact name takahiro fujimoto
E-mail(s) [email protected]
Organization name fukuoka university
Department Faculty of medicine,Department of cell biology
Street address 7-45-1 nanakuma,jonan-ku
City Fukuoka
State/province Fukuoka
ZIP/Postal code 814-0180
Country Japan
 
Platform ID GPL1261
Series (2)
GSE13582 Expression data from BAT of the KRAP deficient mice
GSE13585 Expression data from BAT and liver of the KRAP deficient mice
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE For each transcript represented on the probe array, the algorithm computes a Signal.
ABS_CALL For each transcript represented on the probe array, the algorithm computes a Detection call.
DETECTION P-VALUE For each transcript represented on the probe array, the algorithm computes a Detection p-value.

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 201.254 P 0.000146581
AFFX-BioB-M_at 330.232 P 4.42873e-05
AFFX-BioB-3_at 193.169 P 4.42873e-05
AFFX-BioC-5_at 604.676 P 5.16732e-05
AFFX-BioC-3_at 707.311 P 5.16732e-05
AFFX-BioDn-5_at 1630.55 P 4.42873e-05
AFFX-BioDn-3_at 3122.95 P 4.42873e-05
AFFX-CreX-5_at 7157.4 P 4.42873e-05
AFFX-CreX-3_at 7601.91 P 4.42873e-05
AFFX-DapX-5_at 87.1451 P 5.16732e-05
AFFX-DapX-M_at 284.844 P 0.000340305
AFFX-DapX-3_at 553.943 P 4.42873e-05
AFFX-LysX-5_at 19.4413 P 0.000972149
AFFX-LysX-M_at 43.8912 P 0.00499819
AFFX-LysX-3_at 150.559 P 9.4506e-05
AFFX-PheX-5_at 15.3436 P 0.0396608
AFFX-PheX-M_at 28.3228 P 0.0012475
AFFX-PheX-3_at 59.238 P 0.000126798
AFFX-ThrX-5_at 30.1289 P 0.00401721
AFFX-ThrX-M_at 54.5119 P 0.000169227

Total number of rows: 45101

Table truncated, full table size 1377 Kbytes.




Supplementary file Size Download File type/resource
GSM341695.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM341695.CHP.gz 894.7 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file
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