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Sample GSM341696 Query DataSets for GSM341696
Status Public on Jan 22, 2009
Title Expression data from BAT of the KRAP deficient mice (WT3)
Sample type RNA
 
Source name brown adipose tissue
Organism Mus musculus
Characteristics C57BL6J-backcrossed, male
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA followed by purification using Rneasy kit was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description WT3-BAT
Data processing The data were analyzed with GeneChip Operating Software (GCOS) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Nov 13, 2008
Last update date Aug 28, 2018
Contact name takahiro fujimoto
E-mail(s) [email protected]
Organization name fukuoka university
Department Faculty of medicine,Department of cell biology
Street address 7-45-1 nanakuma,jonan-ku
City Fukuoka
State/province Fukuoka
ZIP/Postal code 814-0180
Country Japan
 
Platform ID GPL1261
Series (2)
GSE13582 Expression data from BAT of the KRAP deficient mice
GSE13585 Expression data from BAT and liver of the KRAP deficient mice
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE For each transcript represented on the probe array, the algorithm computes a Signal.
ABS_CALL For each transcript represented on the probe array, the algorithm computes a Detection call.
DETECTION P-VALUE For each transcript represented on the probe array, the algorithm computes a Detection p-value.

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 235.323 P 0.000296708
AFFX-BioB-M_at 311.658 P 4.42873e-05
AFFX-BioB-3_at 201.211 P 4.42873e-05
AFFX-BioC-5_at 708.55 P 5.16732e-05
AFFX-BioC-3_at 749.633 P 4.42873e-05
AFFX-BioDn-5_at 1705.78 P 4.42873e-05
AFFX-BioDn-3_at 3104 P 4.42873e-05
AFFX-CreX-5_at 8474.18 P 5.16732e-05
AFFX-CreX-3_at 8885.73 P 4.42873e-05
AFFX-DapX-5_at 95.4176 P 6.02111e-05
AFFX-DapX-M_at 302.682 P 0.000389797
AFFX-DapX-3_at 440.019 P 4.42873e-05
AFFX-LysX-5_at 17.3668 P 0.000389797
AFFX-LysX-M_at 39.3322 P 0.00933744
AFFX-LysX-3_at 91.5063 P 0.000146581
AFFX-PheX-5_at 28.3106 P 0.000855711
AFFX-PheX-M_at 34.4819 P 0.00141043
AFFX-PheX-3_at 66.2162 P 0.000972149
AFFX-ThrX-5_at 41.9055 P 0.00618711
AFFX-ThrX-M_at 58.5002 P 0.00010954

Total number of rows: 45101

Table truncated, full table size 1379 Kbytes.




Supplementary file Size Download File type/resource
GSM341696.CEL.gz 3.5 Mb (ftp)(http) CEL
GSM341696.CHP.gz 890.9 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file
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