|
| Status |
Public on Nov 25, 2008 |
| Title |
sechellia_bodies_NC_1 |
| Sample type |
RNA |
| |
|
| Source name |
NO CHOICE
|
| Organism |
Drosophila sechellia |
| Characteristics |
Tissue: Adult Female Bodies
|
| Growth protocol |
reared at 25° C on agar-yeast-cornmeal medium with constant humidity in an environmental chamber
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from heads or bodies was extracted using TRIzol reagent as per manufacturer instructions. RNA was further purified using a Qiagen RNeasy Mini Kit per the manufacturer’s protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
|
| Description |
Adult females bodies
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Nov 24, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ian Dworkin |
| Organization name |
Michigan State University
|
| Department |
Zoology
|
| Street address |
203 Natural Science Bldg. Michigan State University
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE13723 |
D. simulans vs. Sechellia bodies choice vs. no choice -Affymetrix |
|
| Relations |
| Reanalyzed by |
GSE119084 |
