Origin of sample: Sciatic nerve pooled from 4 to 7 mice, 4 days old (P4) and carrying a Pmp22 transgene (Magyar, J.P., et al., Impaired differentiation of Schwann cells in transgenic mice with increased Pmp22 gene dosage. J. Neurosci. 16, 5351-5360 (1996)). RNA extracted with RNeasy kits (Qiagen). Target preparation by two rounds of in vitro transcription (Hoffmann, R., Seidl, T., Neeb, M., Rolink, A. & Melchers, F. Changes in gene expression profiles in developing B cells of murine bone marrow. Genome Res 12, 98-111 (2002)). Labeling protocol: Standard Affymetrix biotin labeling protocol (Affymetrix, Expression Analysis: Technical manual, 2000) in 2nd round of in vitro transcription. Hybridization procedure: Standard Affymetrix protocols (Affymetrix, Expression analysis: Technical manual, 2000). Scanning with Affymetrix scanner and MAS 5.0 software. The original images (.dat files) are available on request ([email protected]) The .cel files produced by MAS 5.0 were normalized using the program dChip (smoothing spline normalization; Li, C. & Wong, W.H. Model-based analysis of oligonucleotide arrays: model validation, design issues and standard error application. Genome Biol. 2, research0032.0031-0032.0011 (2001); http://www.dchip.org). dChip was also used to calculate a probe sensitivity index for each probe on the basis of 27 arrays, and then a "model-based expression index" (MBEI) for each gene on each array (Li, C. & Wong, W.H. Model-based analysis of oligonucleotide arrays: expression index computation and outlier detection. Proc. Natl. Acad. Sci. U.S.A. 98, 31-36 (2001)). Keywords = peripheral nervous system Keywords = PMP22
