|
| Status |
Public on Jun 30, 2009 |
| Title |
01MLR_PolyRF+ |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood mononuclear cells, RF+ polyarthritis
|
| Organism |
Homo sapiens |
| Characteristics |
TTF=103min
Race=Caucasian/White
Sex=Female
Group=A
Prototype_N
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Peripheral blood was drawn and PBMC were isolated over Ficoll. Samples were stored in Trizol at -80oC until extracted according to manufacturer's directions.
|
| Label |
Biotin
|
| Label protocol |
NuGEN Ovation labeling
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol
|
| Scan protocol |
Standard scanning
|
| Description |
01MLR_PolyRF+
|
| Data processing |
Data were imported into GeneSpring 7.3 with RMA preprocessing and normalized to median of all samples. Normalized data were exported and subjected to Distance Weighted Discrimination (DWD) to account for any batch effects then reimported into Gene Spring and analyzed.
|
| |
|
| Submission date |
Dec 05, 2008 |
| Last update date |
May 03, 2010 |
| Contact name |
Michael Barnes |
| E-mail(s) |
[email protected]
|
| Phone |
513-636-1319
|
| Organization name |
Cincinnati Childrens Hospital Medical Center
|
| Department |
Rheumatology
|
| Lab |
Dr. David Glass
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE13849 |
Expression Signatures in Polyarticular JIA Show Heterogeneity and Offer a Molecular Classification of Disease Subsets |
| GSE21521 |
Immature cell populations and an erythropoiesis gene expression signature in systemic juvenile idiopathic arthritis: Implications for pathogenesis |
|
