|
| Status |
Public on Sep 30, 2009 |
| Title |
B cells_Postmenopausal_Smoking_high BMD_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
B cells in blood
|
| Organism |
Homo sapiens |
| Characteristics |
Postmenopause, smoking
High BMD
|
| Treatment protocol |
B cells isolated from 70mL of whole blood using a B cells positive method Dynabeads CD19 (Pan B)Kit (Invitrogen Life Technologies Dynal Biotech Inc.,CA) following manufacturer's instructions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from B cells was extracted using Qiagen Rneasy Mini Kit (Qiagen Inc.,CA).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
The microarray was washed with Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from high BMD postmenopausal female smoker
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) and RMA.
|
| |
|
| Submission date |
Dec 05, 2008 |
| Last update date |
Jan 02, 2009 |
| Contact name |
Peng Xiao |
| E-mail(s) |
[email protected]
|
| Phone |
402-280-4283
|
| Fax |
402-280-4284
|
| Organization name |
Creighton University Medical Center
|
| Department |
Biomedical Sciences & Osteoporosis Research Center
|
| Lab |
Genetic Lab
|
| Street address |
601 N 30th ST Suite 6730
|
| City |
Omaha |
| State/province |
NE |
| ZIP/Postal code |
68131 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE13850 |
Gene Expression of Circulating B Lymphocytes for Smoking-related Osteoporosis in Postmenopausal Females |
|
