|
| Status |
Public on May 08, 2019 |
| Title |
Spleen_transformed_Bcell_myeloma-like_Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
B cell_transformed_MYC+BMI1+BCLXL+IRF4
|
| Organism |
Mus musculus |
| Characteristics |
sample type: C57BL/6_spleen_LPS-stimulated_Bcell_transformed
strain: C57BL/6
|
| Treatment protocol |
Cells were transduced with pools of retroviral particles on day 2 and 3 after LPS stimulation. Two different pools or retroviruses were used. Samples B13-1, B13-2 and B13-3 were transduced with retroviral parrticles encoding MYC, BMI1 and BCLXL. Samples named P7- 1, P7-2, P7-3 and P7-4 were transduced with retroviral particles encoding MYC, BMI1, BCLXL and IRF4. After transduction, the cells were kept in culture between 6 - 12 weeks before extraction of RNA. Each sample are obtained from individual preparations of spleen cells, transduced with independent preparations of retroviruses. The samples B13-1, B13-2 and B13-3 are three biological replicates. The samples P7- 1, P7-2, P7-3 and P7-4 are four biological replicates.
|
| Growth protocol |
Spleen cells from normal untreated C57BL/6 mice were stimulated with LPS (25 microgram/ml) in vitro.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
The Ambion WT Expression kit (Life Technologies, Carlsbad, CA; P/N 4411974) were used together with the Affymetrix GeneChip WT Terminal Labeling kit.
|
| |
|
| Hybridization protocol |
Hybridization, washing and scanning of the GeneChip Mouse Gene 1.1 ST 16-Array Plate were performed on a GeneTitan Instrument according to the manufacturer’s recommendations.
|
| Scan protocol |
Arrays were scanned on an Affymetrix GeneTitan instrument.
|
| Description |
Cells similar to transformed plasma cells or myeloma cells
|
| Data processing |
Affymetrix Expression console used following summarization: Plier, Norm.: Global Median
|
| |
|
| Submission date |
Nov 24, 2018 |
| Last update date |
May 08, 2019 |
| Contact name |
Jessica Margareta Lindvall |
| E-mail(s) |
[email protected]
|
| Organization name |
SciLifeLab. Stockholm University
|
| Department |
NBIS
|
| Street address |
Tomtebodavägen 23A
|
| City |
Solna |
| ZIP/Postal code |
11111 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE122883 |
Malignant transformation of normal mature mouse B cells into myeloma-like cells in vitro using defined genetic elements |
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