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Sample GSM3487647 Query DataSets for GSM3487647
Status Public on May 08, 2019
Title Spleen_transformed_Bcell_myeloma-like_Rep3
Sample type RNA
 
Source name B cell_transformed_MYC+BMI1+BCLXL+IRF4
Organism Mus musculus
Characteristics sample type: C57BL/6_spleen_LPS-stimulated_Bcell_transformed
strain: C57BL/6
Treatment protocol Cells were transduced with pools of retroviral particles on day 2 and 3 after LPS stimulation. Two different pools or retroviruses were used. Samples B13-1, B13-2 and B13-3 were transduced with retroviral parrticles encoding MYC, BMI1 and BCLXL. Samples named P7- 1, P7-2, P7-3 and P7-4 were transduced with retroviral particles encoding MYC, BMI1, BCLXL and IRF4. After transduction, the cells were kept in culture between 6 - 12 weeks before extraction of RNA. Each sample are obtained from individual preparations of spleen cells, transduced with independent preparations of retroviruses. The samples B13-1, B13-2 and B13-3 are three biological replicates. The samples P7- 1, P7-2, P7-3 and P7-4 are four biological replicates.
Growth protocol Spleen cells from normal untreated C57BL/6 mice were stimulated with LPS (25 microgram/ml) in vitro.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol The Ambion WT Expression kit (Life Technologies, Carlsbad, CA; P/N 4411974) were used together with the Affymetrix GeneChip WT Terminal Labeling kit.
 
Hybridization protocol Hybridization, washing and scanning of the GeneChip Mouse Gene 1.1 ST 16-Array Plate were performed on a GeneTitan Instrument according to the manufacturer’s recommendations.
Scan protocol Arrays were scanned on an Affymetrix GeneTitan instrument.
Description Cells similar to transformed plasma cells or myeloma cells
Data processing Affymetrix Expression console used following summarization: Plier, Norm.: Global Median
 
Submission date Nov 24, 2018
Last update date May 08, 2019
Contact name Jessica Margareta Lindvall
E-mail(s) [email protected]
Organization name SciLifeLab. Stockholm University
Department NBIS
Street address Tomtebodavägen 23A
City Solna
ZIP/Postal code 11111
Country Sweden
 
Platform ID GPL11533
Series (1)
GSE122883 Malignant transformation of normal mature mouse B cells into myeloma-like cells in vitro using defined genetic elements

Data table header descriptions
ID_REF
VALUE Linear signal after PLIER normalisation

Data table
ID_REF VALUE
10338001 1675.063416
10338002 12.21007367
10338003 515.5612416
10338004 282.0877097
10338005 5.063026376
10338006 5.617779503
10338007 5.815890069
10338008 6.408559021
10338009 44.63179732
10338010 4.993322196
10338011 8.876555777
10338012 5.278031643
10338013 4.75682846
10338014 4.924577653
10338015 4.993322196
10338016 24.7610399
10338017 3929.145833
10338018 13.73704698
10338019 8.224910613
10338020 38.05462768

Total number of rows: 35556

Table truncated, full table size 562 Kbytes.




Supplementary file Size Download File type/resource
GSM3487647_M3_F07.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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