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Sample GSM3507826 Query DataSets for GSM3507826
Status Public on Dec 12, 2018
Title Mixture_Sample_3
Sample type SRA
 
Source name Mixture_Sample_3
Organism Homo sapiens
Characteristics origin: cell line
sample name: Mixture_3
tumor-mcf7 proportions: 85
cafs-hs_343.t proportions: 3
normal_breast-hmecs-htert proportions: 9
lymphocytes-namalwa proportions: 3
treatment: control
Treatment protocol No treatment
Growth protocol MCF7 cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS). Namalwa cells were a gift from Dr. Sandeep Dave, Duke University, and were cultured in RPMI medium supplemented with 10% FBS. Hs343T and hTERT-HME1 (ATCC) were cultured in HuMEC Ready medium (Thermo Fisher Scientific).
Extracted molecule polyA RNA
Extraction protocol RNeasy kit
TruSeq RNA sample preparation kit v2
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description all_samples_per_gene_counts.txt
Data processing Generation of FASTQ files performed using Illumina bcl2fastq2
FASTQ files from each sequencing run concatenated into single file per library
Adapter sequence trimmed using cutadapt 1.12
Trimmed reads aligned using STAR version 2.5.2b to GRCh38 assembly
Counts per gene determined using featureCounts version 1.5.1 based on Ensembl release 90 GRCh38 annotations
Coverage tracks generated using genomeCoverageBed (bedtools v2.25.0)
Genome_build: GRCh38
Supplementary_files_format_and_content: bigWig (.bw) containing raw read coverage; tab-delimited text (.txt) containing raw read counts per gene for all samples
 
Submission date Dec 11, 2018
Last update date Dec 14, 2018
Contact name Kai Kang
E-mail(s) [email protected]
Organization name MIT
Department CSAIL
Street address 32 Vassar Street
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL18573
Series (1)
GSE123604 A novel computational complete deconvolution method using RNA-seq data
Relations
BioSample SAMN10579031
SRA SRX5124485

Supplementary file Size Download File type/resource
GSM3507826_Mixture_Sample_3_coverage.bw 137.8 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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