|
| Status |
Public on Apr 13, 2020 |
| Title |
WT_25_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
wild type (Hu2185) withnduction at 25°C for 4 hours
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
treatment: Induction at 25 degrees C for 4 hours
strain: Hu2185
genotype: wildtype
growth phase: mid-log exponential phase
|
| Treatment protocol |
Wild type (Hu2185) and abo1∆ strain (Hu2318) were cultured to mid-log exponential phase and continuously induced at 25°C, 30°C and 37°C for 4 hours.
|
| Growth protocol |
Standard YES medium was used for cell culture. Standard fission yeast genetic techniques and media were used according to Moreno et al (1991). Yeast cells were grown to mid-logarithmic phase.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were then harvested by centrifugation at 3000 rpm and lysed by incubation with TES buffer (10mM Tris-HCl, pH 7.5, 10mM EDTA and 0.5% SDS). RNA was extracted by using hot acid-phenol method
|
| Label |
biotin
|
| Label protocol |
Samples were prepared and hybridized to GeneChip S.pombe Tiling 1.0FR Array according to Affymetrix specifications
|
| |
|
| Hybridization protocol |
Samples were prepared and hybridized to GeneChip S.pombe Tiling 1.0FR Array according to Affymetrix specifications
|
| Scan protocol |
The tiling arrays were scanned using an Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Processed data files:
WT_25_signal_normalized.bar
WT_25_R_signal_normalized.bar
Result file is normalized from the two replicates and mapped to the reverse strans of pombe genome (Sanger 2007)
|
| Data processing |
The signal raw data files were normalized through Affymetrix Tiling analysis Software (TAS)
The replicate result was first normalized and merged into one file. The normalized microarray data were mapped to S. pombe genome (Sanger 2007).
Results files are provided as .bar and .bar.txt files.
|
| |
|
| Submission date |
Jan 30, 2019 |
| Last update date |
Apr 13, 2020 |
| Contact name |
Wenbo Dong |
| E-mail(s) |
[email protected]
|
| Phone |
0046769797414
|
| Organization name |
Karolinska Institutet
|
| Department |
Biosciences and Nutrition
|
| Lab |
Andreas Lennartsson
|
| Street address |
Hälsovägen 7C Neohuset
|
| City |
Hudidnge |
| State/province |
Sweden |
| ZIP/Postal code |
14183 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL7715 |
| Series (2) |
| GSE125910 |
Abo1 is required for H3K9me2 to H3K9me3 transition in telomeric and centromeric heterochromatin [gene expression] |
| GSE125912 |
Abo1 is required for H3K9me2 to H3K9me3 transition in telomeric and centromeric heterochromatin |
|
