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| Status |
Public on Jan 08, 2020 |
| Title |
307 cell vector control |
| Sample type |
SRA |
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| Source name |
mammary tumor cell line
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| Organism |
Mus musculus |
| Characteristics |
celltype: mammary tumor primary cell line
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Kit (Qiagen, Hilden, Germany)
TruSeq Stranded Total RNA Library Prep Kit
following manufacturer user guide from Illumina
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Description |
SAM24323319
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| Data processing |
Illumina Casava 1.8
Sequencing reads were filtered to have at least an average base quality of 23 and were then aligned to GRCm38 using GSNAP
Expression counts per gene were obtained by counting the number of reads aligned uniquely to each gene locus as defined by NCBI and Ensembl gene annotations and RefSeq mRNA sequences
Genome_build: GRCm38
Supplementary_files_format_and_content: Excel file with gene expression counts
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| Submission date |
Feb 11, 2019 |
| Last update date |
Jan 08, 2020 |
| Contact name |
Steffen Durinck |
| E-mail(s) |
[email protected]
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| Organization name |
Genentech
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| Street address |
1 DNA Way
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| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platform ID |
GPL17021 |
| Series (2) |
| GSE126350 |
study CRISPR/Cas9 knock down line by RNA-seq |
| GSE126351 |
CRISPR/Cas9 knock down line |
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| Relations |
| BioSample |
SAMN10909063 |
| SRA |
SRX5358042 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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