|
| Status |
Public on Apr 01, 2019 |
| Title |
H1299 PBS biological replica 2 |
| Sample type |
RNA |
| |
|
| Source name |
NCI-H1299
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: NCI-H1299
tumor type: lung carcinoma
treatment: PBS
|
| Treatment protocol |
Cells were plated and after overnight incubation, vehicle or 12.8 µM Omomyc treatement were added
|
| Growth protocol |
Cells were grown at 37ºC, 5% CO2 in RPMI1640 supplemented with glutamine and 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using TRIzol following manufacturer's procedures. Total RNA was quantified using a NanoDrop Spectrophotometer ND-1000 (NanoDrop Technologies, Inc.) and its integrity was assessed using a 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
Biotin
|
| Label protocol |
Labeling of samples was carried out according to the manufacturer’s standard protocol
|
| |
|
| Hybridization protocol |
Total RNA was linearly amplified using WT Plus (Affymetrix). The resulting ssDNA was used to hybridize Affimetrix microarrays. Hybridization data was acquired using the Affimetrix GeneChip/GeneTitan platforms. Transcriptomes were determined on a genome wide Clariom S Human HT (Affimetrix).
|
| Scan protocol |
Arrays were scanned on a Affymetrix GeneTitan instrument (ThermoFisher Scientific-Affymetrix) according to the manufacturer’s protocol.
|
| Data processing |
Affymetrix CEL files were normalized by RMA preprocessing methodology and analyzed using Partek Genomic Suite followed by GSEA 3.0 software from Broad Institute.
|
| |
|
| Submission date |
Feb 12, 2019 |
| Last update date |
Apr 02, 2019 |
| Contact name |
Marie-Eve Beaulieu |
| E-mail(s) |
[email protected]
|
| Organization name |
Peptomyc
|
| Street address |
Natzaret 115-117
|
| City |
Barcelona |
| State/province |
Catalunya |
| ZIP/Postal code |
08035 |
| Country |
Spain |
| |
|
| Platform ID |
GPL24324 |
| Series (2) |
| GSE126453 |
Effect of Omomyc treatment on human lung adenocarcinoma cell lines |
| GSE126455 |
Effect of Omomyc treatment on lung adenocarcinoma of KRasLSL-G12D/+ transgenic mouse and human lung adenocarcinoma cell lines |
|
