|
| Status |
Public on Feb 17, 2020 |
| Title |
S2 Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
S2
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype/variation: wild type
cell line: S2 cells
|
| Treatment protocol |
Cells were treated with 1mM CuSo4 for 24h.
|
| Growth protocol |
S2 cells were grown on Schneider’s insect Medium (Biowest) supplemented with 100 μg/ml streptomycin (Gibco), 100 units/ml penicilin (Gibco) and 10% FBS (Gibco). dBigH1::FLAG cells were maintained in Schneider's insect medium (Biowest) supplmented with 0.3 mg/ml Hygromycin B (Sigma) and for performing the experiments cells were grown on the same medium as S2 cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Mini kit (Qiagen) according to manufacturer's recommendations.
|
| Label |
biotin
|
| Label protocol |
25 ng total RNA per sample was processed using isothermal amplification SPIA Biotin System (NuGEN Technologies, Inc.)
|
| |
|
| Hybridization protocol |
2.2 µg of cDNA was hybridized per microarray for 16 hours of hybridization at 45ºC, washing and staining of microarrays was performed using a Fluidics Station 450 (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
GeneChips were scanned in a GeneChip Scanner 3000 (Affymetrix, Santa Clara, CA)
|
| Description |
date: 3/28/2018
gene expression from wild type S2 cells
|
| Data processing |
Data was quantile normalized and RMA summarized using Bioconductor.
|
| |
|
| Submission date |
Feb 26, 2019 |
| Last update date |
Feb 17, 2020 |
| Contact name |
Oscar Reina Garcia |
| E-mail(s) |
[email protected]
|
| Organization name |
IRB Barcelona
|
| Department |
Biostatistics and Bioinformatics
|
| Street address |
C/Baldiri Reixac 10
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE127226 |
The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin [Affy] |
| GSE127227 |
The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin |
|
