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Sample GSM366758 Query DataSets for GSM366758
Status Public on May 01, 2009
Title MCF7 cells E2 Stimulated 12hr replicate 2
Sample type RNA
 
Source name MCF7 cells
Organism Homo sapiens
Characteristics MCF7 cells E2 Stimulated 12hr replicate 2
Extracted molecule total RNA
Extraction protocol Total RNA was collected using Trizol (Invitrogen, Paisley, UK). After extraction the quality and levels of RNA was determined using an Agilent Bioanalyzer (Agilent Technologies, Edinburgh, UK) and a NanoDrop® ND-1000 Spectrophotometer (Thermo Scientific, Horsham, UK)
Label cy3
Label protocol Isolated RNA was labelled using the Agilent labelling kit following the manufacturer’s instruction (Agilent Technologies)
 
Hybridization protocol Agilent standard protocol
Scan protocol Microarrays were scanned with Agilent scanner G2565BA using Agilent Feature Extraction software
Description none
Data processing Raw data from the Agilent feature extraction software.
 
Submission date Feb 03, 2009
Last update date Feb 08, 2009
Contact name geraint barton
E-mail(s) [email protected]
Organization name Imperial College
Department Bioinformatics Support Service
Street address South Kensington Campus
City London
ZIP/Postal code sw7 2az
Country United Kingdom
 
Platform ID GPL6955
Series (1)
GSE14685 Genome wide screen of estrogen receptor α regulated microRNAs reveals a negative autoregulatory feedback loop

Data table header descriptions
ID_REF
gIsFound
VALUE gProcessedSignal
gProcessedSigError
gNumPixOLHi
gNumPixOLLo
gNumPix
gMeanSignal
gMedianSignal
gPixSDev
gPixNormIQR
gBGNumPix
gBGMeanSignal
gBGMedianSignal
gBGPixSDev
gBGPixNormIQR
gNumSatPix
gIsSaturated
gIsLowPMTScaledUp
gIsFeatNonUnifOL
gIsBGNonUnifOL
gIsFeatPopnOL
gIsBGPopnOL
IsManualFlag
gBGSubSignal
gBGSubSigError
gIsPosAndSignif
gPValFeatEqBG
gNumBGUsed
gIsWellAboveBG
gBGUsed
gBGSDUsed
ErrorModel
gSpatialDetrendIsInFilteredSet
gSpatialDetrendSurfaceValue
SpotExtentX
SpotExtentY
gNetSignal
gTotalProbeSignal
gTotalProbeError
gTotalGeneSignal
gTotalGeneError
gIsGeneDetected
gProcessedBackground
gProcessedBkngError
IsUsedBGAdjust
gInterpolatedNegCtrlSub
gIsInNegCtrlRange

Data table
ID_REF gIsFound VALUE gProcessedSigError gNumPixOLHi gNumPixOLLo gNumPix gMeanSignal gMedianSignal gPixSDev gPixNormIQR gBGNumPix gBGMeanSignal gBGMedianSignal gBGPixSDev gBGPixNormIQR gNumSatPix gIsSaturated gIsLowPMTScaledUp gIsFeatNonUnifOL gIsBGNonUnifOL gIsFeatPopnOL gIsBGPopnOL IsManualFlag gBGSubSignal gBGSubSigError gIsPosAndSignif gPValFeatEqBG gNumBGUsed gIsWellAboveBG gBGUsed gBGSDUsed ErrorModel gSpatialDetrendIsInFilteredSet gSpatialDetrendSurfaceValue SpotExtentX SpotExtentY gNetSignal gTotalProbeSignal gTotalProbeError gTotalGeneSignal gTotalGeneError gIsGeneDetected gProcessedBackground gProcessedBkngError IsUsedBGAdjust gInterpolatedNegCtrlSub gIsInNegCtrlRange
1 1 1.99E+02 2.04E+01 0 1 63 3.28E+02 323 3.31E+01 3.04E+01 697 8.48E+01 84 1.15E+01 1.19E+01 0 0 0 0 0 0 0 0 199.35 20.3806 1 0 1 1 129.047 11.5298 2 0 129.047 52.6241 52.6241 296.077 154.339 15.8221 154.339 15.8221 1 84.8077 11.5298 0 204.32 0
2 1 7.78E+00 4.31E+00 2 0 62 1.36E+02 133.5 1.58E+01 1.63E+01 525 8.21E+01 82 1.11E+01 1.11E+01 0 0 0 0 0 0 0 0 7.77898 4.30935 1 2.26E-06 1 0 128.173 11.1307 2 0 128.173 48.5334 48.5334 103.632 17.5894 7.7915 17.5894 7.7915 0 82.0857 11.1307 0 12.5533 0
3 1 4.69E+00 4.26E+00 0 0 65 1.32E+02 129 1.86E+01 2.00E+01 535 8.11E+01 81 1.06E+01 1.19E+01 0 0 0 0 0 0 0 0 4.68763 4.2644 1 0.00416693 1 0 127.328 10.6311 2 0 127.328 49.8279 49.8279 99.6954 -0.848725 2.8116 -4.09302 3.97355 0 81.0935 10.6311 0 9.29302 1
4 1 1.44E+02 1.50E+01 0 0 65 2.70E+02 266 3.42E+01 4.30E+01 514 7.88E+01 79 1.08E+01 1.11E+01 0 0 0 0 0 0 0 0 143.559 14.9685 1 0 1 1 126.488 10.8291 2 0 126.488 49.1849 49.1849 237.726 100.131 10.4103 128.478 11.1502 1 78.7821 10.8291 0 147.974 0
5 1 3.43E+00 4.25E+00 1 0 64 1.29E+02 129 1.41E+01 1.50E+01 518 7.76E+01 77 1.01E+01 1.02E+01 0 0 0 0 0 0 0 0 3.43361 4.25244 0 0.0355686 1 0 125.723 10.0778 2 0 125.723 47.2035 47.2035 96.8363 1.05314 2.82033 0.919246 3.98435 0 77.5907 10.0778 0 7.71592 1
6 1 -6.23E+00 4.24E+00 1 0 63 1.19E+02 120 1.22E+01 1.41E+01 523 7.80E+01 77 1.09E+01 1.11E+01 0 0 0 0 0 0 0 0 -6.23348 4.23855 0 0.000144164 1 0 125.011 10.8715 2 0 125.011 49.5074 49.5074 86.4578 -3.01154 2.80785 -5.39695 3.9709 0 78.0459 10.8715 0 -2.04187 1
7 1 -3.25E+00 4.24E+00 1 1 60 1.21E+02 122 1.32E+01 1.26E+01 527 7.80E+01 77 1.05E+01 1.04E+01 0 0 0 0 0 0 0 0 -3.25342 4.23855 0 0.0463853 1 0 124.303 10.4769 2 0 124.303 48.5334 48.5334 88.73 -0.457231 1.4057 -3.12751 2.81026 0 77.9715 10.4769 0 0.835524 1
8 1 -5.92E+00 4.24E+00 1 0 64 1.18E+02 118 1.52E+01 1.50E+01 512 7.81E+01 78 1.09E+01 1.11E+01 0 0 0 0 0 0 0 0 -5.91515 4.23855 0 0.000307613 1 0 123.603 10.8693 2 0 123.603 49.1849 49.1849 85.3675 1.23419 1.42755 0.763922 2.82345 0 78.1406 10.8693 0 -1.93998 1
10 1 2.28E+00 4.24E+00 0 0 65 1.24E+02 126 1.80E+01 1.85E+01 513 7.68E+01 77 1.10E+01 1.11E+01 0 0 0 0 0 0 0 0 2.28068 4.24469 0 0.16229 1 0 122.212 10.958 2 0 122.212 49.5074 49.5074 92.1723 8.07149 11.2805 8.07149 11.2805 0 76.7661 10.958 0 5.98495 1
11 1 2.00E+00 4.24E+00 1 0 66 1.24E+02 124.5 1.58E+01 1.46E+01 541 7.60E+01 75 1.11E+01 1.19E+01 0 0 0 0 0 0 0 0 1.99588 4.24325 0 0.221283 1 0 121.519 11.1438 2 0 121.519 45.4864 45.4864 91.1952 -0.381199 1.4044 1.07729 2.817 0 76.0333 11.1438 0 5.54608 1
12 1 -2.38E+00 4.24E+00 0 0 64 1.18E+02 117.5 1.86E+01 1.89E+01 520 7.47E+01 74 1.05E+01 1.11E+01 0 0 0 0 0 0 0 0 -2.38407 4.23855 0 0.14411 1 0 120.822 10.4844 2 0 120.822 49.8279 49.8279 86.1175 1.0309 11.2627 1.0309 11.2627 0 74.6519 10.4844 0 0.990859 1
14 1 5.18E+00 4.27E+00 1 0 63 1.25E+02 123 1.72E+01 1.67E+01 518 7.43E+01 74 9.77E+00 9.64E+00 0 0 0 0 0 0 0 0 5.18368 4.27013 1 0.00154438 1 0 119.435 9.76765 2 0 119.435 47.2035 47.2035 92.299 1.41571 1.41168 4.78113 2.82868 0 74.3475 9.76765 0 8.17593 1
17 1 -3.21E+00 4.24E+00 0 0 64 1.14E+02 114.5 1.37E+01 1.35E+01 510 7.31E+01 73 9.55E+00 9.64E+00 0 0 0 0 0 0 0 0 -3.20578 4.23855 0 0.0496704 1 0 117.44 9.55491 2 0 117.44 48.2044 48.2044 81.9144 -1.67341 1.40393 -4.02703 2.81042 0 73.1098 9.55491 0 -0.836174 1
19 1 1.38E+01 4.46E+00 2 1 61 1.30E+02 128 1.60E+01 1.70E+01 524 7.46E+01 74 1.06E+01 1.11E+01 0 0 0 0 0 0 0 0 13.7545 4.45614 1 2.12E-16 1 0 116.278 10.5531 2 0 116.278 48.2044 48.2044 97.7128 5.4892 1.51692 61.7592 4.37421 1 74.5859 10.5531 0 15.8065 0
20 1 -2.05E+00 4.24E+00 0 0 68 1.14E+02 114 1.88E+01 2.08E+01 522 7.39E+01 73 1.09E+01 1.26E+01 0 0 0 0 0 0 0 0 -2.0503 4.23855 0 0.208958 1 0 115.741 10.9462 2 0 115.741 45.835 45.835 81.3712 -2.04992 2.80828 -3.68904 3.97415 0 73.8716 10.9462 0 -0.134038 1
22 1 9.57E+00 4.35E+00 0 0 63 1.24E+02 122 1.74E+01 2.11E+01 513 7.37E+01 73 1.01E+01 1.04E+01 0 0 0 0 0 0 0 0 9.57162 4.34529 1 6.91E-09 1 0 114.571 10.1154 2 0 114.571 46.5243 46.5243 91.8229 5.20275 1.76709 6.05521 3.30567 0 73.7037 10.1154 0 11.0584 1
23 1 5.69E+00 4.28E+00 1 0 64 1.20E+02 118 1.48E+01 1.43E+01 523 7.30E+01 73 9.73E+00 9.64E+00 0 0 0 0 0 0 0 0 5.68651 4.27653 1 0.000519247 1 0 114.017 9.73193 2 0 114.017 47.5395 47.5395 87.3831 17.0724 11.3907 17.0724 11.3907 0 73.0172 9.73193 0 6.96449 1
26 1 -4.73E+00 4.24E+00 1 0 65 1.08E+02 107 1.47E+01 1.48E+01 529 7.31E+01 73 9.93E+00 1.04E+01 0 0 0 0 0 0 0 0 -4.72656 4.23855 0 0.00386609 1 0 112.373 9.92516 2 0 112.373 49.1849 49.1849 75.3262 -0.487287 1.40393 -2.40477 2.80988 0 73.0662 9.92516 0 -4.14565 1
27 1 2.74E+00 4.25E+00 0 0 66 1.15E+02 115 1.31E+01 1.41E+01 518 7.43E+01 74 1.04E+01 1.04E+01 0 0 0 0 0 0 0 0 2.73727 4.24738 0 0.0936181 1 0 112.02 10.4148 2 0 112.02 49.1849 49.1849 82.4376 0.177605 1.40639 -0.513764 2.81309 0 74.2799 10.4148 0 3.25133 1
28 1 6.60E+01 7.84E+00 1 0 61 1.78E+02 178 2.55E+01 2.59E+01 527 7.28E+01 72 1.05E+01 1.11E+01 0 0 0 0 0 0 0 0 65.9519 7.83977 1 2.38E-179 1 1 111.835 10.4913 2 0 111.835 48.5334 48.5334 145.467 44.0329 5.23923 129.03 10.3766 1 72.7533 10.4913 0 66.5498 0

Total number of rows: 12033

Table truncated, full table size 3046 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
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