|
| Status |
Public on Dec 31, 2019 |
| Title |
MCF7 DMSO, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
treatment: None
|
| Treatment protocol |
MCF7 cells were treated with 1 μM AF for 8h or 5 μM Onc1 for 16h.
|
| Growth protocol |
MCF7 cells were maintained in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum and penicillin/streptomycin (10 units/ml)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with the Aurum total RNA mini kit (Bio-Rad) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on HT-Human Genome U133+ PM Array.
|
| Scan protocol |
GeneChips were scanned using a confocal scanner (Affymetrix).
|
| Description |
MCF7 control
|
| Data processing |
The data were analyzed with geneXplain platform, and RMA method was used for background correction, normalization, and summarization.
|
| |
|
| Submission date |
Mar 14, 2019 |
| Last update date |
Jan 01, 2020 |
| Contact name |
Sylvain Peuget |
| Organization name |
Karolinska Institute
|
| Department |
MTC
|
| Street address |
Solnavägen 9
|
| City |
Solna |
| State/province |
Stockholm |
| ZIP/Postal code |
17165 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE128329 |
Expression profiling of MCF7 cells upon Aminoflavone (AF) or Oncrasin1 (Onc1) treatment |
|
