|
| Status |
Public on May 10, 2021 |
| Title |
postnatal skeletal muscle at P4, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Rat 4-day old skeletal muscle
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar rat
age: 4-day old newborn
tissue: skeletal muscle
|
| Treatment protocol |
The fetuses were delivered by Caesarean section after sacrifice of pregnant Wistar rat mothers. Adult P90 controls were collected. All tissues were snap frozen in liquid nitrogen immediately. Tissues were stored for further analyses at -80 °C.
|
| Growth protocol |
The animals were kept and all rat tissues were obtained in the Department of Genetics of Model Diseases, Institute of Physiology CAS (Prague, Czech Republic). The experiments were performed in agreement with the Animal Protection Law of the Czech Republic and were approved by the Ethics Committee of the Institute of Physiology AS CR (Prague, Czech Republic).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriReagent extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5.5 ug total RNA (RIN>8) using Ambion WT kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 °C on Rat Gene 1.0 ST Array. Arrays were scanned according to the protocol described in WT Sense Target Labeling Assay Manual (FS450_0007).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
RaGene1_120313W_JZ22_B4
RaGene1_120313W_JZ01_B4
Gene expression data from neonatal skeletal muscle tissue, fourth postnatal day, suckling period.
|
| Data processing |
The data were analyzed with Expression console, using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
May 10, 2019 |
| Last update date |
May 10, 2021 |
| Contact name |
Jana Krizova |
| E-mail(s) |
[email protected]
|
| Organization name |
Charles University in Prague, First Faculty of Medicine, and General University Hospital in Prague
|
| Department |
Department of Pediatrics and Inherited Metabolic Disorders
|
| Lab |
Laboratory for Study of Mitochondrial Disorders
|
| Street address |
Ke Karlovu 2
|
| City |
Prague 2 |
| State/province |
Non United States Or Canada |
| ZIP/Postal code |
12808 |
| Country |
Czech Republic |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE131012 |
Microarray and qPCR Analysis of Mitochondrial Metabolism Activation during Prenatal and Early Postnatal Development in Rats and Humans with Emphasis on CoQ10 Biosynthesis |
|
