|
| Status |
Public on Mar 05, 2010 |
| Title |
PR9692-E9-EGR1 metastasizing cell line, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Sarcoma derived PR9692-E9-EGR1 metastatic cell line in culture
|
| Organism |
Gallus gallus |
| Characteristics |
cell line: Sarcoma derived PR9692-E9-EGR1 metastatic cell line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 10 millions cultured cells were dissolved in 2 ml TRIzol reagent and total RNA was extracted according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Chicken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from sarcoma derived PR9692-E9-EGR1 metastatic cell line in culture
|
| Data processing |
The data were analyzed with Bioconductor (version 2.3) using Affymetrix default annotation and GC-RMA as normalization method.
|
| |
|
| Submission date |
Mar 06, 2009 |
| Last update date |
Mar 06, 2009 |
| Contact name |
Robert Ivanek |
| Phone |
+41 61 207 35 41
|
| Organization name |
University of Basel
|
| Department |
Department of Biomedicine
|
| Lab |
Bioinformatics
|
| Street address |
Hebelstrasse 20
|
| City |
Basel |
| ZIP/Postal code |
4053 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE15141 |
EGR1 regulates metastatic potential of v-src transformed chicken sarcoma cell lines |
|
