|
| Status |
Public on Mar 05, 2010 |
| Title |
Primary skin fibroblasts infected with retroviral construct SFCVneo-EGR4 replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Cultured primary skin fibroblasts isolated from 10 days old chicken embryos and infected with SFCVneo-EGR4 retroviral construct.
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: primary skin fibroblast
age: embryo, 10 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 3 millions (subconfluent 10cm plate) of cultured cells were dissolved in 2 ml TRIzol reagent and total RNA was extracted according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Chicken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from cultured primary skin fibroblasts isolated from 10 days old chicken embryos and infected with SFCVneo-EGR4 retroviral construct.
|
| Data processing |
The data were analyzed with Bioconductor (version 2.3) using Affymetrix default annotation and GC-RMA as normalization method.
|
| |
|
| Submission date |
Mar 06, 2009 |
| Last update date |
May 04, 2012 |
| Contact name |
Vladimir Cermak |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Molecular Genetics, Czech Academy of Sciences
|
| Lab |
Molecular Virology
|
| Street address |
Videnska 1083
|
| City |
Prague |
| ZIP/Postal code |
14220 |
| Country |
Czech Republic |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE15143 |
An Antifibrotic Regulatory Network Governed by the Transcription Factor EGR4 |
|
