|
| Status |
Public on May 23, 2019 |
| Title |
Ctrl Normoxia [177] |
| Sample type |
RNA |
| |
|
| Source name |
Non-targeting siRNA treated and maintained in 21% oxygen
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hASC differentiated to adipocytes
sirna: Non-targeting
condition: 21% oxygen
|
| Treatment protocol |
40 nM siRNA delivered with HiperFect. Oxygen levels were reduced in an hypoxic chamber.
|
| Growth protocol |
13 days with adipogenic differentiation cocktail in the media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using a column based RNA extraction kit (from Machery-Nagel).
|
| Label |
biotin
|
| Label protocol |
Not provided
|
| |
|
| Hybridization protocol |
Not provided
|
| Scan protocol |
Not provided
|
| Description |
177
|
| Data processing |
SST-RMA (TAC)
|
| |
|
| Submission date |
May 22, 2019 |
| Last update date |
May 23, 2019 |
| Contact name |
Paul Petrus |
| E-mail(s) |
[email protected]
|
| Organization name |
Karolinska intitute
|
| Lab |
Lipidlab
|
| Street address |
Hälsovägen 7
|
| City |
Stockholm |
| ZIP/Postal code |
14157 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE131667 |
CD248 knockdown in in vitro differentiated adipocytes exposed to hypoxia |
|
