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Sample GSM3912178 Query DataSets for GSM3912178
Status Public on Dec 10, 2019
Title Liver, WT Chow 1
Sample type SRA
 
Source name Liver
Organism Mus musculus
Characteristics strain: C57BL/6
age: 5 months
genotype: WT
treatment: Chow
tissue: liver
Extracted molecule total RNA
Extraction protocol Livers were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Zymogen Research Direct-zol RNA Miniprep Plus (Cat#R2072) was used. 2 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using Illumina TruSeq Stranded RNA Kit wit Ribo-Zero Gold
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1000
 
Description CHOW1
Data processing Sequenced reads were trimmed for adaptor sequence, and masked for low-quality sequence, then mapped to mm10 whole genome using CLCBio Genomics Workbench 11 with default parameters
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text file includes counts, FPKM values for each Sample
 
Submission date Jul 01, 2019
Last update date Dec 10, 2019
Contact name Yuxia Zhang
E-mail(s) [email protected]
Phone 9135881749
Organization name University of Kansas Medical Center
Department Pharmacology, Toxicology, and Therapeutics
Street address 3901 Rainbow Blvd, Hemenway 4001
City kansas city
State/province KS
ZIP/Postal code 66160
Country USA
 
Platform ID GPL15103
Series (1)
GSE133566 Shp-deletion induces dissociation of steatosis and inflammation in NASH
Relations
BioSample SAMN12169334
SRA SRX6384935

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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