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Status |
Public on Dec 10, 2019 |
Title |
Liver, WT Chow 2 |
Sample type |
SRA |
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Source name |
Liver
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Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 age: 5 months genotype: WT treatment: Chow tissue: liver
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Extracted molecule |
total RNA |
Extraction protocol |
Livers were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Zymogen Research Direct-zol RNA Miniprep Plus (Cat#R2072) was used. 2 ug of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using Illumina TruSeq Stranded RNA Kit wit Ribo-Zero Gold
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 1000 |
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Description |
CHOW2
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Data processing |
Sequenced reads were trimmed for adaptor sequence, and masked for low-quality sequence, then mapped to mm10 whole genome using CLCBio Genomics Workbench 11 with default parameters Genome_build: mm10 Supplementary_files_format_and_content: tab-delimited text file includes counts, FPKM values for each Sample
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Submission date |
Jul 01, 2019 |
Last update date |
Dec 10, 2019 |
Contact name |
Yuxia Zhang |
E-mail(s) |
[email protected]
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Phone |
9135881749
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Organization name |
University of Kansas Medical Center
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Department |
Pharmacology, Toxicology, and Therapeutics
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Street address |
3901 Rainbow Blvd, Hemenway 4001
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City |
kansas city |
State/province |
KS |
ZIP/Postal code |
66160 |
Country |
USA |
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Platform ID |
GPL15103 |
Series (1) |
GSE133566 |
Shp-deletion induces dissociation of steatosis and inflammation in NASH |
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Relations |
BioSample |
SAMN12169345 |
SRA |
SRX6384936 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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