|
| Status |
Public on Apr 16, 2009 |
| Title |
Human osteoblast sample 576 repl 2 |
| Sample type |
RNA |
| |
|
| Source name |
Primary human osteoblasts
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Trabecular bone
gender: female
|
| Growth protocol |
The cells were grown in medium containing α-MEM supplemented with 2 mmol/l L-Glutamine, 100U/mL penicillin, 100mg/mL streptomycin and 10% fetal bovine serum at 37°C with 5% CO2. At 70-80% confluence, the cells were passaged and sub-cultured in 6-well plates (100,000 cells/well) for 12 days after which the cells were harvested and stored at -70°C until RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cell lysates resuspended in 600µl RLT lysis buffer using the RNeasy® Mini Kit. High RNA quality was confirmed for all samples using the Agilent 2100 BioAnalyzer and the concentrations were determined using Nanodrop ND-1000.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
none
|
| Data processing |
The data were normalized using variance-stabilizing transformation and robust spline normalization
|
| |
|
| Submission date |
Apr 15, 2009 |
| Last update date |
Apr 15, 2009 |
| Contact name |
Elin Grundberg |
| E-mail(s) |
[email protected]
|
| Organization name |
McGill University and Genome Quebec Innovation Centre
|
| Street address |
740 Dr Penfield Avenue
|
| City |
Montreal |
| ZIP/Postal code |
H3A1A4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE15678 |
Population genomics in a disease targeted primary cell model |
|
