|
| Status |
Public on Aug 04, 2020 |
| Title |
GEP-NETs Patient3_G1 |
| Sample type |
RNA |
| |
|
| Source name |
Tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Gastroenteropancreatic neuroendocrine tumors (GEP-NETs)
sample tissue: FFPE tissue
gender: Male
age: 73
tumor grade: G1
tumor localization: Duodenum
|
| Treatment protocol |
No treatment
|
| Growth protocol |
No growth
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA, including small RNA fraction, was isolated from FFPE section of 5 µm-thickness using miRNeasy FFPE kit (Qiagen) according to the manufacturer’s protocol. Total RNA was then eluted in ribonuclease-free water. The RNA concentration was determined with NanoDrop ND-2000 Spectrophotometer (Nanodrop Technologies).
|
| Label |
Cy3
|
| Label protocol |
The miRNA expression levels were assessed using SurePrint G3 Human Microarray Agilent Human 8x60K miRNA Microarray (Agilent Technologies) based on Sanger miRBase release 21, according to the manufacturer’s instructions. Briefly, 100 ng of total RNA isolated from FFPE section of 18 NENs patients were firstly dephosphorylated with a calf intestine alkaline phosphatase treatment for 30 minutes at 37°C before labeling. Samples were diluted with DMSO, denatured for 10 minutes at 100°C, and labeled using pCp-Cy3 in T4 RNA ligation buffer.
|
| |
|
| Hybridization protocol |
According to the Agilent miRNA Microarray Protocol (Agilent Technologies).
|
| Scan protocol |
After hybridization and washes, slides were scanned immediately in Agilent microarray scanner (G2565BA, Agilent)
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3 (Agilent) using default parameters.
|
| |
|
| Submission date |
Jul 29, 2019 |
| Last update date |
Aug 04, 2020 |
| Contact name |
Grazia Serino |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institute of Gastroenterology “S. de Bellis”, Research Hospital
|
| Street address |
Via Turi, 27
|
| City |
Castellana Grotte (BA) |
| ZIP/Postal code |
70013 |
| Country |
Italy |
| |
|
| Platform ID |
GPL21576 |
| Series (1) |
| GSE135034 |
microRNA profiling in different grading of gastroenteropancreatic neuroendocrine tumors (GEP-NETs) |
|
