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Sample GSM399367 Query DataSets for GSM399367
Status Public on May 02, 2009
Title T.4int8+.Th#1
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4int 8+ TCRhi
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass Slide Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.4int8+.Th#1, Positive Selection, Intermediate
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.4168
10344622 266.686
10344624 363.6539
10344633 739.5644
10344637 222.9558
10344653 15.6083
10344658 220.9596
10344674 11.1442
10344679 35.942
10344707 255.5726
10344713 320.6534
10344723 221.6882
10344725 48.49
10344741 3343.2865
10344743 22.4427
10344750 478.3207
10344772 27.5798
10344789 173.3174
10344797 290.6955
10344799 248.4295

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399367_EA07068_56651_MoGene_T.4int8+.Th_#1.cel.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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