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Sample GSM399377 Query DataSets for GSM399377
Status Public on May 02, 2009
Title T.4SP69+.Th#2
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8- TCRhi 69+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.4SP69+.Th#2, CD4 Single-Positive, Intermediate
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSE73864

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 15.3497
10344622 237.3543
10344624 391.3799
10344633 692.093
10344637 187.7137
10344653 20.6408
10344658 195.2733
10344674 11.4004
10344679 34.9692
10344707 189.1559
10344713 352.8907
10344723 216.0872
10344725 45.3459
10344741 2404.9473
10344743 28.1265
10344750 497.1195
10344772 28.8207
10344789 113.9617
10344797 363.1544
10344799 162.093

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399377_EA07068_52769_MoGene_T.4SP69+.Th_#2.cel.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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